Experiments in molecular cell biology

Zusatztext

Preface Types of multiple-choice questions 11. Restriction mapping of a plasmid DNA 12. Restriction mapping of a linear DNA fragment 13. Cutting and ligating l phage DNA 14. Construction of a subtractive genomic library 15. Sequencing of DNA using the Maxam-Gilbert method 16. DNA fingerprinting with a minisatellite probe in a family 17. In vitro synthesis of infectious phage DNA 18. Replication of SV40 virus DNA 19. In vitro synthesis of Okazaki fragments 10. Proofreading by bacterial DNA polymerase I 11. Functional analysis of a DNA repair protein 12. The phases of cell cycle 13. Regulation of lamin function by protein kinases 14. In vitro transcription by a bacterial RNA polymerase 15. Isolation of RNA polymerases from frog cells 16. mRNA stability 17. Polysomes and protein synthesis 18. Analysis of protamine-synthesizing polysomes 19. Protein translocation across the endoplasmic reticulum membrane 20. The role of calnexin in the secretory pathway 21. Analysis of the lac-repressor 22. Lactose operon mutants 23. Regulation of gene expression in mitogen-activated lymphocytes 24. The role of the 3'-untranslated region in mRNA metabolism 25. Sucrosomes 26. Mitochondrial protein transport 27. Purification of tubulins 28. SCG10, a cytoskeleton-associated protein 29. Consequences of the DF508 cystic fibrosis gene mutation 30. Signal transduction from the epidermal growth factor receptor 31. The regulation of a mitogen-actived protein kinase 32. The effect of nerve growth factor on an early-response gene 33. Targeted gene disruption 34. Catalytic activity of the SV40 T antigen 35. Identification of the v-src gene product 36. Analysis of the mutation that causes Burkitt lymphoma 37. The role of p53 protein in the cellular response to g-irradiation 38. Analysis of a tumor by comparative genomic hybridization 39. Pedigree of hemophilia A 40. Colour blindness and Klinefelter syndrome 41. Inheritance of nail-patella syndrome and ABO blood groups