Plant Gene Containment

Gene Containment provides a comprehensive look at genetically modified organisms and the strategies and implementation of key methods to gene containment. The book is divided into 5 parts: An Introduction that discusses the need for biotechnology and GMOs, Section 1 looks at the need for gene containment, Part II discusses varying strategies for gene containment, section III explores the assessment of gene containment approaches, and section IV covers the steps involved in implementing gene containment. Gene Containment will provide a thorough and up to date look at gene containment research and the needs for implementing new strategies in this arena.

InhaltsangabeList of Contributors ix Foreword xi J. Perry Gustafson and Peter H. Raven Section 1 Perspectives of Gene Flow 1 Chapter 1 Potential Environmental Impacts of Transgene Flow in Rice with a Particular View on Herbicide Resistance 3 BaoRong Lu and Wei Wang Introduction 3 Transgene Escape and Its Potential Environmental Impacts 4 HerbicideResistant Rice 8 Gene Flow from Herbicide-Resistant Rice and Its Potential Impacts 12 Managing Potential Environmental Impacts from Transgene Flow 17 Conclusion 19 Acknowledgments 20 References 20 Chapter 2 Assessment and Detection of Gene Flow 27 Wei Wei, Charles Kwit, Reginald J. Millwood, Hong S. Moon, and C. Neal Stewart Jr. Importance of Assessment and Detection of Gene Flow 27 Detection Methodologies 29 Applications in Assessment of Gene Flow 35 Perspectives 36 Acknowledgments 37 References 37 Chapter 3 Bt Resistance Monitoring in European Corn Borers and Western Corn Rootworms 43 Blair D. Siegfried and Terence Spencer Introduction 43 Resistance Monitoring 44 Bioassay Methods and Baseline Susceptibility 47 Recent Developments 49 Conclusions 51 Acknowledgments 52 References 52 Chapter 4 Commercialization of GMOs: the Company Perspective on Risks and Containment 57 Kater Hake Commercialization of Traits Derived from Plant Biotechnology (GMOs and Alien Chromosome Introgression) 57 Gene Containment During the Commercial Utilization of Crops Derived from Biotechnology 60 Gene Containment During Research, Development, and Seed Production 62 Stewardship and Gene Containment 63 References 65 Section 2 Strategies for Gene Containment 67 Chapter 5 Trait Control (Gene Switch Technology) 69 M. Isabel Ordiz, Jaemo Yang, and Roger N. Beachy Components of Gene Switch Systems 69 Application of Gene Switch Technologies 75 Future Perspectives 79 References 80 Chapter 6 Male Sterility and Hybrid Plant Systems for Gene Confinement 85 Albert P. Kausch, Joel Hague, Adam Deresienski, Michael Tilelli, and Kimberly Nelson Introduction 85 Cytoplasmic Male Sterility 86 Transgenic Nuclear Male Sterility 87 Transgenic Nuclear Male Sterility for Gene Confinement 89 Male Sterility for Recovery of Wide Crosses with Gene Confinement 90 Hybrid Plant Systems 92 SeedBased Hybrid Plant Systems 92 GeneDeletor System 92 Male and Female Sterile Lines 94 Total Sterility 95 Stable Knockout Mutations 96 References 96 Chapter 7 Gene-Deletor Technology and Its Potential Applications in Addressing Gene Flow and Food Safety Concerns over Transgenic Plants 101 Yi Li, Hui Duan, Yongqin Chen, and Richard McAvoy Introduction 101 The Principle of Gene-Deletor Technology and Supporting Experimental Data 102 Characteristics and Requirements of Gene-Deletor Technology 105 Potential Applications of Gene-Deletor Technology 107 Conclusions and Perspectives 109 Acknowledgments 110 References 110 Chapter 8 Seed-Based Gene Containment Strategies 113 Melvin J. Oliver and Kater Hake Seedless Crops 114 SeedBased Containment 115 GeneSafe (Technology Protection System (TPS)) Technology 117 Expression Cascades for Gene Containment 120 References 123 Chapter 9 Transgenic Mitigation of Transgene Dispersal by Pollen and Seed 125 Jonathan Gressel and Hani Al-Ahmad IntroductionContainers Leak 125 MitigationThe Concept of Conferring a Selective Disadvantage Outside of Cultivation 128 Evidence That TM Mitigates Transgene Establishment 133 Tailoring Mitigator Genes to Crops and Cropping Situation 137 Concluding Remarks 142 References 142 Chapter 10 Tapping RNA Silencing for Transgene Containment through the Engineering of Sterility in Plants 147 Zongrang Liu and Stacy D. Singer Introduction 147 References 166 Chapter 11 Control of Cleistogamy and Seed Dormancy for Biological Gene Containment in Oilseed Rape (Brassica napu

Leseprobe